Preparation of Low Molecular Weight Glucomannan from A. Konjac K. Koch in Vietnam by Enzyme Catalyzed Hydrolysis Reaction and its Prospective use to Lower Blood Sugar Levels-Juniper Publishers
JUNIPER PUBLISHERS- ACADEMIC JOURNAL OF POLYMER SCIENCE Abstract In order to break down the glycoside bond in glucomannan obtained from the bulb of the Konjac in Việt Nam, we used the extract from bacteria bacillus subtilis. The reaction was carried out at 40oC for 6h, pH 5 and the E/S ratio of 0.4(w/w). The obtained oligo-glucomannan after hydrolysis that had molecular weight lower than 1748Da was tested for glucose tolerence in experimental animals. The test results showed that the hydrolyzed glucomannan at a dose of 6g/kg was more effective in lowering blood glucomannan in mouse models that were made to have blood sugar increase exogenously by oral admistration, as compared to original glucomannan. Keywords: Hydrolyzed glucomannan; Enzyme; Blood sugar Introduction Glucomannan is a water-soluble polysaccharide consisting of D-mannose and D-glucose units linked with β - (1→ 4) glucosidic bonds, with the degree of branching of a...